M3949-01A
MIG (Monokine induced by Interferon Gamma)
Size
£435.00
- SKU:
- M3949-01A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human MIG, expressed in E. coli.
- Clone:
- 7F7
- Format:
- Supplied as a lyophilized powder from PBS. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Human MIG (monokine induced by interferon-g) is produced by macrophages and other cells and is a member of the a-chemokine family (C-X-C) of cytokines. MIG acts as a chemoattractant toward monocytes, lymphocytes, and certain T cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (sandwich): 2-4ug/ml (100ul/well) detects at least 0.2ng/well of recombinant human MIG when used with M3949-03 as the detection antibody. Western Blot: 0.50-2.0ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant human MIG is 0.25-0.50ng/lane, under non-reducing conditions and 2.0-4.0ng/lane, under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice