M3895-07C

Migration Inhibitory Factor (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)

Size

£863.00

SKU:: M3895-07C
Application:: WB
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Pro2-Ala115 of human Migration Inhibitory Factor expressed in E. coli.
Clone:: 2Ar3
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Extra Details:: MIF is a ubiquitous protein that is found in all cells and can be released under various conditions. MIF has intrinsic thiol-protein oxidoreductase activity. It was also reported to be a mediator of immune and inflammatory conditions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using K562 human chronic myelogenous leukemia cell line Simple Western: 10ug/ml using THP-1 human acute monocytic leukemia cell line or U937 human histiocytic lymphoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.