M3895-07B
Migration Inhibitory Factor (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF) (Biotin)
Size
£960.00
- SKU:
- M3895-07B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Pro2-Ala115 of human Migration Inhibitory Factor, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of macrophages. Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of imflammation and immunity. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. HL-60 human acute promyelocytic leukemia cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin. Western Blot: 0.1ug/ml. Recombinant human MIF. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS Aliquot to avoid repeated freezing and thawing. Store at -20°C. . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Ambient