M3891-01A
Microphthalmia (Transcription Factor, MiTF)
Size
£607.00
- SKU:
- M3891-01A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 301-312 of human Mitf.
- Clone:
- 4H205
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3-5 ug/ml Optimal dilutions to be determined by the researcher. Recommended Positive Control: A375 or RAW Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice