M3891-01
Microphthalmia (Transcription Factor, MiTF)
Size
£895.00
- SKU:
- M3891-01
- Application:
- IHC-Fr
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- N-terminal fragment of human Mi protein
- Clone:
- D5
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3891-01X
- Extra Details:
- Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.1 MW of Antigen: 52-56kD doublet Epitope: N-terminal Applications and Recommended Dilutions: Gel Supershift: Order Ab at 1mg/ml. See M3891-01X Western Blotting (Not verified) Immunohistochemistry (Formalin/paraffin: 4-8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Positive Control: 501 Mel cells1. Melanoma Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice