M3890-31A
b2-Microglobulin (b2M)
Size
£787.00
- SKU:
- M3890-31A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A/G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ile21-Met119 from rat B2-Microglobulin expressed in NS0 cell line.
- Clone:
- 12K137
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- B2 Microglobulin (B2M) is a ubiquitous, 12kD, secreted, non-glycosylated protein required for cell surface expression and noncovalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat B2M is a 99aa peptide containing one C1-type lg-like domain (aa22-116). In humans, B2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal truncated peptides of 93, 91, and 90aa. Mature rat B2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 rat splenocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice