M3890-05A

b2-Microglobulin (B2M, Beta-2-microglobulin) (HRP)

Size

£800.00

SKU:: M3890-05A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Highly purified beta 2 microglobulin from human urine
Clone:: 12K274
Format:: Supplied as a liquid in PBS, pH 7.2, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Extra Details:: b2-microglobulin non-covalently associates with the 44kD a-chain to form the HLA Class I antigen complex. Human b2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Matched Pair: Capture: M3890-01U Dectection: M3890-05A Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.