M3890-01Z

b2-Microglobulin (b2M)

Size

£678.00

SKU:: M3890-01Z
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Highly purified recombinant Human Beta-2 MG.
Clone:: D3A2
Format:: Supplied as a lyophilized powder from a solution in 0.01M PBS, pH 7.2.
Extra Details:: Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119aa, chromosome 15). B2M associates with heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml coated antigen can be detected by ≥ 1ng/ml of antibody Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O, PBS, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice