M3889-25
Microcystin LR
Size
£626.00
- SKU:
- M3889-25
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
- Clone:
- 6D584
- Format:
- Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS, sodium azide.
- Extra Details:
- With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 Concentrations can be measured in the range of ~0.006ug/L - 0.3ug/L.. The ELISA sensitivity is about 1000 times higher compared to UV detection. Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice