M3887-62H

MHC Class 2, I-Ek (Biotin)

Size

£479.00

SKU:: M3887-62H
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: C3H mouse skin graft and splenocytes
Clone:: 14-4-4S
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 Applications: • Flow cytometry1 • Complement-dependent cytotoxicity1 • Immunoprecipitation3 • In vitro induction of antigen-specific B-cell differentiation4 • In vitro blocking of antigen presentation5 • Immunohistochemistry (acetone-fixed, frozen tissue sections)6 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.