M3887-55C
MHC Class 2 (beta chain) (FITC)
Size
£557.00
- SKU:
- M3887-55C
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified MHC class II from JY cell line.
- Clone:
- 7H218 (TDR31.1)
- Format:
- Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride. 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17aa MHC Class II molecules interact with the T cell receptor on CD4+ T cells. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml) labels 5x10e5 human Daudi cells Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x10e5 cultured human Daudi cells were incubated on ice for 45 minutes with 80ul of M3887-55C at a 1:50 dilution factor (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.92 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A MHC Class 2 (beta chain). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice