M3887-55B
MHC Class 2 (beta chain) (Biotin)
Size
£557.00
- SKU:
- M3887-55B
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified class II from JY cell line.
- Clone:
- 7H218 (TDR31.1)
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17 amino acids. MHC Class II molecules interact with the T cell receptor on CD4+ T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Flow Cytometry: 10ug/ml labels 5x10e5 cultured human Daudi cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Daudi cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (To block non specific binding). 2. After which they were incubated for 45 minutes on ice with 80ul of M3887-55B at 10ug/ml. 3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE) after which they were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 2.71 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml M3887-55A: MHC Class 2 (beta chain). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice