M3887-55A
MHC Class 2 (beta chain)
Size
£479.00
- SKU:
- M3887-55A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified class II from JY cell line
- Clone:
- 7H218 (TDR31.1)
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human MHC Class II (HLA-DP, DQ & DR) molecules are expressed on dendritic cells, monocytes, macrophages, and myeloid cells. MHC Class II molecules are heterodimers of polymorphic transmembrane a and b chains and generally bind exogenously derived peptides of about 10-17 amino acids. MHC Class II molecules interact with the T cell receptor on CD4+ T cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Daudi human tumor cells were incubated for 45 minutes on ice with 80ul of M3887-55A at 5ug/ml. 2. Cells were washed twice and incubated with 2º reagent Goat anti-Mouse IgG/FITC. 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 2.16 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Recommended Secondary Antibodies: I1904-76L IgG1 (FITC) Pab Gt xMo I1904-79L IgG1 (FITC) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice