M3887-53D

MHC Class 2, I-Ak (H-2 Class II Histocompatibility Antigen, A-K alpha Chain, H2-Aa, H-2 Class II Histocompatibility Antigen, A-K beta Chain, H2-Ab1)

Size

£518.00

SKU:: M3887-53D
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: CKB mouse splenocytes
Clone:: 11-5.2
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: This antibody reacts with the alpha chain of the I-Ak MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. This antibody cross-reacts with cells from mice bearing the H-2r haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. This antibody has been reported to block antigen presentation and induce B cell differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤1ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.