M3887-49M

RT1B (MHC Class II Monomorphic)

Size

£563.00

SKU:: M3887-49M
Purity:: Purified by Protein G affinity chromatography from from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat thymocyte membrane glycoprotein
Clone:: 3H440 (OX-6)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Paraffin): 1:50-1:100. Requires antigen retrival using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6 is recommended. PLP fixation is recommended. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.