M3887-49M-BIOTIN
RT1B (MHC Class II Monomorphic) (Biotin)
Size
£852.00
- SKU:
- M3887-49M-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat thymocyte membrane glycoprotein
- Clone:
- 3H440 (OX-6)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100 Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): 1:50-1:100. Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer, pH 6.0 is recommended. Immunohistochemistry: Frozen sections Optimal dilution determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice