M3886-55C-PE

MHC Class 1, H-2Dd (MHC Class I, H-2Dd) (PE)

Size

£672.00

SKU:: M3886-55C-PE
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: (C57BL/6 x DBA/2)F1 mouse splenocytes.
Clone:: 34-2-12
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: This antibody recognizes a3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. This clone recognizes the a3 domain of Dd. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.