M3886-55C-FITC
MHC Class 1, H-2Dd (MHC Class I, H-2Dd) (FITC)
Size
£639.00
- SKU:
- M3886-55C-FITC
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- (C57BL/6 x DBA/2)F1 mouse splenocytes.
- Clone:
- 34-2-12
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- This antibody recognizes a3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. This clone recognizes the a3 domain of Dd. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice