M3886-07-FITC
MHC Class 1, HLA G (FITC)
Size
£1,081.00
- SKU:
- M3886-07-FITC
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Denatured bacterially expressed recombinant human HLA G heavy chain.
- Clone:
- 3H2680 (MEM-G/1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region.HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Western Blot: Reducing conditions Optimal dilutions to be determined by researcher. Recommended Positive Control Tissue: Human placenta - extravillous cytotrophoblast Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice