M3885-73R
MHC Class 1, H2, Kd, Dd
Size
£408.00
- SKU:
- M3885-73R
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- BDF1 mouse splenocytes
- Clone:
- 34-1-2S
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
- Extra Details:
- MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Complement-mediated cytotoxicity. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: acetone-fixed, frozen tissue sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice