M3885-72D

MHC class I, H-2Kb (MHC Class 1, H-2Kb)

Size

£326.00

SKU:: M3885-72D
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: C57BL mouse splenocytes.
Clone:: AF6-88.5
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: This antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Immunohistochemistry: Acetone-fixed frozen sections. Not suitable for staining of formalin-fixed paraffin embedded sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.