M3885-59C
MHC Class 1, H2, Db, alpha3 (Biotin)
Size
£479.00
- SKU:
- M3885-59C
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- C3H.SW mouse splenocytes
- Clone:
- 28-14-8
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin (3,4). These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells (5). MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny (3,6). This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin (7,8). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Complement-mediated cytotoxicity studies. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice