M3885-59AX

MHC Class 1, H2, Db, alpha3 (Azide Free)

Size

£576.00

SKU:: M3885-59AX
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: C3H.SW mouse splenocytes
Clone:: 28-14-8
Format:: Supplied as a liquid in PBS, pH 7.4 No preservative added.
Extra Details:: MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin.3, 4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3, 6 This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin.7,8 Applications: Suitable in Flow Cytometry, Immunohistochemistry and complement-mediated cytotoxicity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.