M3885-48Q3

MHC Class 1 (MHC Class I) (FITC)

Size

£927.00

SKU:: M3885-48Q3
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified H-2Kb and H-2Db MHC-I molecules.
Clone:: 10F80 (2G5)
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatability 2 (H-2) region. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: X63 myeloma cells with spleen cells from C1D mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.