M3885-48G

MHC Class 1 (PE)

Size

£704.00

SKU:: M3885-48G
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human HSB-2 T cell line
Clone:: 3F10
Format:: Supplied as a liquid in PBS containing 15% glycerol, 0.2% BSA, and 0.04% sodium azide. Labeled with R-Phycoerythrin.
Extra Details:: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution; Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells per test) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.