M3884-79K
mGluR5 (Metabotropic Glutamate Receptor)
Size
£787.00
- SKU:
- M3884-79K
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln21-Ser509 from human mGluR5, expressed in NSO cells.
- Clone:
- 8H205
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150kD, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212aa in length and contains an extracellular domain (ECD) of 558aa. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32aa deletion between aa877-908 in the cytoplasmic tail. Over aa19-509, human mGluR5 is 98% identical to mouse, rat and canine mGluR5. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Intracellular Flow Cytometry: 2.5ug/ml; Use 10ul to label 1-5x10e5 cells in a total reaction volume not exceeding 200ul. Cells must be fixed and permeablized using 4% paraformaldehyde and 0.1% saponin in PBS. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Flow Cytometry: MG-63 cells Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice