mGluR2,3 (Metabotropic Glutamate Receptor)

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£927.00

SKU:: M3884-76
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide (KQFVPTVCNGREVVDSTTSSL) corresponding to aa853-872 of human metabotropic glutamate receptor 2, with a lysine added to the N-terminus for conjugation (KLH).
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%
Extra Details:: Metabotropic glutamate receptors have been classified into three groups. In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission. Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC). Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase. It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects mGluR2/3 in 20ug of rat brain microsomal preparation. Note: Do not boil the microsomal preparation, heat to 37°C for 15-30 minutes. 20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation. Immunohistochemistry (paraffin): Detects mGluR 2/3 in paraffin embedded rat brain tissue sections. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice