M3884-73A
mGluR1 (Metabotropic Glutamate Receptor)
Size
£787.00
- SKU:
- M3884-73A
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa33-522 from human mGluR1, expressed in CHO cells. Species Sequence Homology: mouse, rat, canine; 98%.
- Clone:
- 9L845
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Metabotropic glutamate receptor 1 (mGluR1 a) is a 130kD, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. On neurons, mGluR1 is postsynaptic, associates with G q-like proteins, mobilizes intracellular Ca++, and influences ion channel activity. Mature mGluR1 is 1176aa in length and contains a 574aa N-terminal extracellular domain (ECD) (aa19-592). The ECD binds glutamate and forms either a covalent homodimer, or heterodimer with CaSR. There is one alternative splice form for human mGluR1 that shows a 20aa substitution for the C-terminal 308aa. over aa33-522, human mGluR1 shares more that 98% aa identity with mouse, rat and canine mGluR1. Applications: Suitable for use in Direct ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (caudate putamen). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice