M3884-68

MGL1, MGL2 (Macrophage Galactose N-acetyl-galactosamine Specific Lectin 1, Macrophage Galactose N-acetyl-galactosamine Specific Lectin 2)

Size

£889.00

SKU:: M3884-68
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gln57-Ser304 of mouse MGL1 expressed in NSO cells.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Applications: Suitable for use in Flow Cytometry, Direct ELISA, Inhibition and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 use 10ul to label 10e6 cells Raw264 mouse monocyte macrophage cell line Western Blot: 0.1ug/ml Recombinant mouse MGL1, MGL2 ELISA (Inhibition): 0.4-1.6ug/ml Will block 50% of the binding of 500ng/ml of biotinylated Lex-Polyacrylamide to immobilized recombinant mouse MGL1 coated at 2.5µg/ml (100ul/well). At 15ug/ml, this antibody will block >90% of the binding. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.