mFLIP, CT (FLICE Inhibitory Protein)

Size

£655.00

SKU:: M3750
Application:: WB
Purity:: Purified IgG
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa (2,6).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT (3-8). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. This polyclonal antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. NIH/3T3 whole cell lysate can be used as positive control and a 55kD band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and this antibody recognizes the FLIPL only.