M3398-55M
5-Methylcytidine
Size
£927.00
- SKU:
- M3398-55M
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Ovalbumin-conjugated 5-methylcytosine
- Clone:
- 10B11 (33D3)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- 5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT's). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues. Applications: Suitable for use in Dot Blot, Immunofluorescence, Flow Cytometry, Southern Blot, Methylated DNA Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Dot Blot: 1ug/ml detected methylcytosine in methylated and unmethylated BRCA1. Optimal dilutions to be determined by the researcher. Control: Methylated and unmethylated BRCA1 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice