M3015-98

Methionine Aminopeptidase 1 (MetAP-1)

Size

£812.00

SKU:: M3015-98
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to His52-Phe386 of human Methionine Aminopeptidase 1 derived from Sf21 cells. Species sequence homology: chimpanzee 99%; rat/mouse 98%; chicken 95%; drosophila 64%
Clone:: 7H228
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Methionine Aminopeptidase 1 (MetAP-1) encodes a cytoplasmic protease that removes the NH 2-terminal Met from nascent proteins. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with recombinant human methionine aminopeptidase 1 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.