M3009-60
Meteorin
Size
£800.00
- SKU:
- M3009-60
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gly22-Asp291 from mouse Meteorin, expressed in NS0 cells.
- Clone:
- 7H112
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Meteorin is a novel 31kD, secreted protein that is expressed by undifferentiated neural and glial progenitors, and astrocytes. Its name derives from it ability to generate glial cells with long meteor-like tails. Mouse meteorin is synthesized as a 291 amino acid (aa) precursor that contains a 270 aa mature region. It contains eight cysteines with no identifiable domains or N-linked glycosylation sites. There is one alternate splice form where the first 164 aa of the precursor are deleted and aa # 165 is changed to a Met, possibly to serve in translation initiation. Mature mouse meteorin is 82% and 77% aa identical to human and bovine meteorin, respectively. Applications: Suitable for use in Western Blot, Immunocytochemistry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml using Recombinant Mouse Meteorin Immunocytochemistry: 8-25ug/ml using immersion fixed 7 day differentiated rat cortical stem cells Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice