M3007-16R
c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Size
£767.00
- SKU:
- M3007-16R
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Hepatocyte growth factor receptor (HGF R), also known as c-MET, is a receptor tyrosine kinase that consists of an extracellular alpha chain and a longer beta chain. The beta chain contains the transmembrane helix and the cytoplasmic portion. Ligand-stimulated phosphorylation of tyrosine 1234 and tyrosine 1235 in HGF R's beta chain results in stimulation of intrinsic kinase activity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/1x10e6 cells using MCF-7 human breast cancer cell line treated with pervanadate, fixed with paraformaldehyde and permealized with methanol. Immunohistochemistry: 5-15ug/ml using immersion fixed, frozen section of mouse embryo. Western Blot: 0.5ug/ml using pervanadate-treated MDA-MB-468 human breast cancer cell line Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice