M3007-13

c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Size

£856.00

SKU:: M3007-13
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Available without azide, BSA and glycerol. See M3007-13A.
Extra Details:: Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor. The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain. Both chains are derived from a single precursor protein by proteolytic cleavage. c-Met is expressed in a wide variety of adult and embryonic tissues. It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF. Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas. c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis. Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:10-1:100 Requires HIER using sodium citrate buffer pH6.0 followed by microwaving for 8-15min prior to staining. Flow Cytometry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice