M3007-06A

c-Met, Cytoplasmic (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Size

£837.00

SKU:: M3007-06A
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the cytoplasmic domain of human c-Met protein
Clone:: 6A125
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide
Extra Details:: The c-Met protein is a receptor tyrosine kinase encoded by the c-Met proto-oncogene. c-Met, also known as HGF receptor or SF receptor, is activated by hepatocyte growth factor (HGF) or scatter factor (SF), and is composed of alpha and beta subunits linked by disulfide bonds. Dysregulated c-Met expression has been detected at both protein and mRNA levels in a variety of human carcinomas and sarcomas. Up to a 2.5-fold increase in mRNA expression has been reported in colorectal tumor tissues, and upregulation of the c-Met protein in association with increasingly malignant behavior has been described in prostate cancer, cutaneous malignant melanoma, and breast cancer. Recent studies using monoclonal Ms anti-c-Met (clone: 3D4) for immunohistochemistry in lymph node-negative breast carcinoma tissues demonstrated that high levels of c-Met expression are associated with poor patient clinical outcome, as assessed over 20-40 years of follow-up. Further, antibodies against the intracellular but not the extracellular domain of c-Met were prognostic, suggesting that overexpression of the cytoplasmic tail of c-Met may play an important role in breast cancer progression. Applications: Suitable for use in Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Epitope retrieval is required using citrate Buffer, pH 6.0. Immunofluorescence (IC): 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice