M3006-01A
MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR)
Size
£863.00
- SKU:
- M3006-01A
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu296-Asp580 from human Mesothelin, expressed in NSO cells.
- Clone:
- 10K75
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Megakaryocyte Potentiating Factor (MPF) is a 33kD soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% aa identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas. Applications: Suitable for use in ELISA, Western Blot and Simple Western. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml under reducing conditions of human ovarian cancer tissue lysates Simple Western: 20ug/ml under reducing conditions of human ovarian cancer tissue lysates and human lung tisses Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice