M2990-50
Merosin (Laminin alpha 2)
Size
£889.00
- SKU:
- M2990-50
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human merosin.
- Clone:
- 2Q598 (5H2)
- Format:
- Supplied as a liquid in buffer. No preservative added.
- Extra Details:
- Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14-19 with multiple developmental abnormalities. Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot, Immunofluorescence, Immunoaffinity Purification and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 50% maximal binding to human merosin at 1:50,000. Immunohistochemistry (Frozen): 1:5000 for staining of 8um acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody. Western Blot: 1:1000 detected Merosin on 10ug of human placental lysate. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Control: Human placenta lysate, vascular breast carcinomas, cultured embryonic retinal neurons. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice