M2965-01L
NF2 (SCH, Neurofibromin 2, Merlin, Schwannomin, Moesin-ezrin-radixin-like Protein, Neurofibromin-2, Schwannomerlin)
Size
£832.00
- SKU:
- M2965-01L
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met363-Lys578 of human NF2 expressed in E. coli.
- Clone:
- 10K76
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- NF2 (Neurofibromin-2; also Merlin) is a 70-75kD tumor suppressor protein whose structure is similar to those proteins belonging to the ERM family of molecules. In general, it participates in contact-mediated cell adhesion, thereby blocking cell proliferation and migration. Human NF2 contains three general regions. There is an N-terminal FERM domain (aa1-302), a central a-helical rod central region (aa303-478), and a unique carboxy-terminal domain (aa479-595). Confluence requires an absence of phosphorylation at Ser516 and Ser10. There are multiple potential splice variants. Four short forms less that 260aa in length lack the aa range used for antigen immunization. There are at least eight additional isoforms that basically show an alternate start site coupled to an 11aa substitution for aa579-595. Over aa363-578, human NF2 shares 97% aa identity with mouse NF2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml Western Blot: 2ug/ml using lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice