M2880-05A

Menin (Multiple Endocrine Adenomatosis 1, MEA1, Multiple Endocrine Neoplasia 1, Men1, Suppressor Candidate Gene 2, SCG2, Wermer Syndrome, Zollinger Ellison Syndrome, ZES)

Size

£787.00

SKU:: M2880-05A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: E. coli-derived rmMEA-1
Clone:: 7H140
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
Extra Details:: MEA-1 is a phylogenetically conserved protein that is highly expressed in primary and secondary spermatocytes and spermatids. It is thought to be essential for embryogenesis, spermatogenesis and sex determination. Mouse MEA-1 shares 99% and 93% amino acid sequence homology with rat and human MEA-1, respectively. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E.coli-derived, recombinant mouse male enhanced antigen 1 (rmMEA-1; aa 1-174: Accession # AAH13344). Applications: Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect mouse MEA-1. Using a colorimetric detection system, the detection limit for rmMEA-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MEA-1. The detection limit for rmMEA-1 is approximately 2 ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice