Melanoma Inhibitory Activity Protein (MIA)

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£576.00

SKU:: M2869-95A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Melanoma Inhibitory Activity Protein expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human Melanoma Inhibitory Activity Protein (M2869-95B) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human Melanoma Inhibitory Activity Protein. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Melanoma Inhibitory Activity Protein is 1.5-3ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry (FFPE): 1-2ug/ml using human pancreas infiltrating ductal adenocarcinoma tissue with an overnight incubation at 4ºC. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were acheived with heat induced antigen retrieval with sodium citrate, pH 6 or enzyme induced antigen retrieval with proteinase K at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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