M2869-04

Melanoma-Associated Antigen

Size

£854.00

SKU:: M2869-04
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Isolated from human melanoma cells.
Clone:: NKI/C-3
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Extra Details:: Recognizes a melanoma-associated antigen present in the cytoplasm of melanoma cells and nevocellular nevi, in contrast to most Mabs against melanoma-associated antigens present in the membrane, which are not retained after formalin fixation. Therefore, the mab is very useful for paraffin sections in routine pathology. Crossreactivity with carcinoids, medullary carcinomas of the thyroid, and occasionally prostatic, primary breast, avarian, and lung carcinomas may be observed. No reaction is seen with basal cell carcinomas or brain tumors. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin sections; no protease pretreatment required): 1:5-1:10; Incubation Time 1 hr at room temperature. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.