MARCH-VII (Axotrophin, MARCH7, membrane-associated ring finger (C3HC4) 7, AXO, AXOT, DKFZP586F1122, MARCH-VII, RNF177)

Size

£642.00

SKU:: M2867-61B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to NFNQESEGRNT at the internal region of human MARCH-VII.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:4000 Western Blot: Preliminary experiments gave an ~125kD band in human spleen and tonsil lysates after 1ug/ml antibody staining. Immunohistochemistry (Paraffin): 3.75ug/ml using human uterus. Steamed antigen retrieval with citrate buffer, pH 6. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.