M2865-15A

Mek1 (MAPK/ERK Kinase 1, MEK 1, MEKK1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, MAPKK1, Prkmk1, ERK Activator Kinase 1)

Size

£512.00

SKU:: M2865-15A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E.coli-expressed mouse full-length MEK1
Format:: Supplied as a liquid in PBS containing 40% glycerol and 0.09% sodium azide.
Extra Details:: MEK1 is one of the MEKs that are upstream of the MAPK ERK1/2. MEK1 is a dual-specific protein kinase that phosphorylates ERK1/2 on both threonine and tyrosine residues in the sequence TEY. Phosphorylation of these residues dramatically activates ERK1/2, which then phosphorylate their downstream substrate. MEK1 contains 393 amino acids and has a calculated molecular weight of 43,470. MEK1 itself is regulated by phos-phorylation by one of the MEK kinases. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunoprecipitation: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.