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M2865-11M

ERK/MAPK, phosphorylated (Thr202/Tyr204)

Size

£761.00

SKU:
M2865-11M
Purity:
Purified by affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK, conjugated to KLH. Species sequence homology: mouse, xenopus, bovine, zebrafish, canine, chicken and monkey.
Format:
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry: 1:100-1:500 Immunocytochemistry: 1:100-1:250 Optimal dilutions determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice