M2865-09G
MEK1 (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)
Size
£367.00
- SKU:
- M2865-09G
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide (KLH-coupled) corresponding to human MEK1.
- Format:
- Supplied as a liquid.
- Extra Details:
- MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Phosphorylation by Mek1 increases Erksí ability to phosphorylate micrtubule-associated proteins and myelin basic proteins. Mek1 is involved in diverse arrays of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis. Mek1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1× TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice