M2865-04B
Mek1, NT (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, Prkmk1, ERK Activator Kinase 1)
Size
£659.00
- SKU:
- M2865-04B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa1-12, MPKKKPTPIQLN(C), near the N-terminal of human Mek1, conjugated to KLH. Species Sequence Homology: hamster and rabbit.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Phosphorylation by Mek1 increases Erksí ability to phosphorylate micrtubule-associated proteins and myelin basic proteins. Mek1 is involved in diverse arrays of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation (3), and DNA recombination during meiosis. Mek1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Colorimetric): 1ug/ml Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Positive Controls: H1838-01: HeLa Cell Lysate, Heat Shocked Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice