M2865-02A1

MEK1, MEK2 (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activated Protein Kinase Kinase 2, MAP Kinase Kinase 2, MAP2K2, MAPKK 2, MKK2, PRKMK2, ERK Activator Kinase 2)

Size

£537.00

SKU:: M2865-02A1
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa200-250 of human MEK1.
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: MEK1 and MEK2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK1/2 is a critical step in the MAP kinase cascade. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.