M2862
Meis 1,2,3
Size
£800.00
- SKU:
- M2862
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- MBP-conjugated fusion protein corresponding to aa60-390 of mouse Meis1.
- Clone:
- 9.2.7
- Format:
- Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
- Extra Details:
- Hox, Pbx, and Meis families of transcription factors form heteromeric complexes and bind DNA through specific homeobox domains. Meis1, 2, and 3 are members of the TALE (three amino acid loop extension) family of homeodomain containing proteins. Meis1 binds to Hox and Pbx proteins to form complexes with distinct DNA-binding specificities. Like Hox and Pbx proteins, the overexpression of Meis1 in BHX-2 myeloid leukemias implies a role for this protein in oncogenesis. Two Meis related proteins, Meis2 and Meis3 (also designated Mrg1 and Mrg2, respectively), possess largely similar sequence identity with Meis1 and express in normal tissues as well as myeloid leukemias. In the pancreas, Meis2 preferentially associates with Pbx1, and together they associate with the pancreas specific homeodomain factor, Pdx1, to repress Pdx1-induced transcriptional activation. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 detects Meis in nuclear extract from K562 cells. K562 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Meis 1/2/3 (1:2,500 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Meis (~54kD and 66kD). Immunohistochemistry: Detects Meis in mouse embryo paraffin sections. Immunoprecipitation: Immunoprecipitates Meis from NIH-3T3 nuclear extract. Optimal dilutions to be determined by the researcher. Positive Control: K562 nuclear extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice