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M2847-58

MED4, Recombinant, Human (Mediator of RNA Polymerase II Transcription Subunit 4, Mediator Complex Subunit 4, Activator-recruited Cofactor 36kD Component, ARC36, DRIP36, FLJ10956, HSPC126, RP11-90M2.2, TRAP/SMCC/PC2 Subunit p36 Subunit, TRAP36, Vitamin D3 Receptor-interacting Protein Complex 36kD Component, VDRIP)

Size

£453.00

SKU:
M2847-58
Application:
WB
Molecular Weight:
30.7
Purity:
~ 80% (SDS-PAGE). Purified by using conventional chromatography techniques.
Storage Conditions:
-20°C
Supplier:
United States Biological
Format:
Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 100mM NaCl, 20% glycerol.
Extra Details:
Mediator complex subunit 4(MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36). This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner. Recombinant human MED4, fused to His-tag at C-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-270 of human MED4 6x His tagged expressed in E. coli. AA Sequence: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice